Plasmids have an essential role to play in a lot of experiments of genetic engineering. We are going to discuss how it is used in genetic engineering. One of the significant plasmids features is that it is multiple cloning sites and it is also the replication of origin, and a market selection. For the process of experiments in Genetic engineering having DNA to be stocked up in test tube environment to the cellular environment, till it is required is necessary. Plasmids are perfect to carry out such experiments with perfection. Also, it will not be wrong to say that the plasmids are the carriers which can be used for storing and studying genes. So before we begin further let us first know about Genetic Engineering and Plasmids in brief.
Genetic engineering is the process in which the scientists perform the modifications of genes. It is a technical process in which the scientists deliberately alter the organism by its genes modification. In simple words, engineering means to design, construct, and optimize certain things. In genetic engineering, the things mentioned above are done with the genes. Through experiments, the scientists can affix the transfer of genes from one gene to another, and they can also transform the genes of an organism to create new genes right from the start and infuse to the other species.
DNA plasmids are the parts of the circular DNA extracted from the chromosomal DNA of the cells of an organism. Plasmids are mostly present in the bacterial cells in which other genes apart from the ones present in the chief chromosomes. It is quite essential as while modifying them you don’t get the main chromosomal DNA altered. DNA with chromosomal has the precise information present that is essential for the modified chromosome and development of bacteria. So there is no negative impact on the organism.
How plasmids are used in genetic engineering:
Plasmids permit to bring a precise assembling of the cell population. This is quite useful as when the scientists experiment the plasmids express interest in the cell, and it becomes more straightforward and more accessible to perform the engineering of the cells. Plasmids are also useful in the process of cloning. So when the scientists performing genetic engineering want to “isolate” a particular gene from the cell and use it for an experiment, they need plasmids. In the process, the scientists amplify the specific gene with PCR. And create the desired results.
Hope you got the idea of how plasmids are used in genetic engineering. If you have any further questions, please feel free to contact us in the comment section.